中药中使用的植物提取物对克氏锥虫的抑制作用。

Inhibition of Trypanosoma cruzi by plant extracts used in Chinese medicine.

作者信息

Lirussi D, Li J, Prieto J M, Gennari M, Buschiazzo H, Ríos J L, Zaidenberg A

机构信息

Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calles 60 y 120, La Plata 1900, Argentina.

出版信息

Fitoterapia. 2004 Dec;75(7-8):718-23. doi: 10.1016/j.fitote.2004.09.017.

DOI:10.1016/j.fitote.2004.09.017

PMID:15567249

Abstract

In this work, we assessed the effect of extracts obtained from 17 plants used in traditional Chinese medicine. These extracts were tested in vitro with the epimastigote form of Trypanosoma cruzi, clone Bra C(15) C(2), at 27 degrees C in F-29 medium at a concentration of 100 microg/ml in axenic cultures. Allopurinol was used as reference drug. Seven plant extracts showed inhibitory activities lower than 25%. Pueraria lobata, Mahonia beaei, Dictamus dasycarpus, Kochia scoparia, Sophora flavescens and Ligustrum lucidum showed effects with inhibition values between 25% and 60%, whereas Lithospermum erythrorhizon, Saussurea lappa, Melia toosendan and Cinnamomum cassia showed the greatest inhibitory activity of 100%. The IC(50) of these extracts were: 0.4, 2.4, 1.8 and 3.9 microg/ml, respectively. The MTT assay was made and did not show cytotoxic activity. These results allowed us to suggest that L. erythrorhizon, S. lappa, M. toosendan and C. cassia could be a source of new compounds against T. cruzi.

摘要

在这项研究中，我们评估了17种中药提取物的效果。这些提取物在体外，于27摄氏度的F - 29培养基中，以100微克/毫升的浓度，对克氏锥虫克隆Bra C(15) C(2)的前鞭毛体形式进行测试。别嘌呤醇用作参考药物。七种植物提取物显示出低于25%的抑制活性。葛根、阔叶十大功劳、白鲜皮、地肤子、苦参和女贞子显示出25%至60%的抑制效果，而紫草、木香、川楝子和肉桂显示出最大100%的抑制活性。这些提取物的半数抑制浓度（IC50）分别为：0.4、2.4、1.8和3.9微克/毫升。进行了MTT检测，未显示出细胞毒性活性。这些结果使我们认为紫草、木香、川楝子和肉桂可能是抗克氏锥虫新化合物的来源。