van Oers Monique M, Abma-Henkens Marleen H C, Herniou Elisabeth A, de Groot Joost C W, Peters Sander, Vlak Just M
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
Greenomics, Plant Research International BV, Wageningen, The Netherlands.
J Gen Virol. 2005 Jul;86(Pt 7):2069-2080. doi: 10.1099/vir.0.80964-0.
The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39.1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named GECCO. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses.
最近从甘蓝夜蛾中分离出的一种单核衣壳核型多角体病毒（ChchNPV）的全基因组序列已被测定。该病毒基因组大小为149622 bp，总体G+C含量为39.1 mol%。该序列包含151个预测的开放阅读框（ORF），最小长度为50个密码子。使用一个新开发的名为GECCO的数据库计算了这些ORF与其他完全测序杆状病毒的ORF的相似性。全基因组的系统发育分析证实了ChchNPV与II组NPV的进化关系，I组NPV特异性gp64基因的缺失也证实了这一点。它是第一个编码增殖细胞核抗原的II组NPV。最值得注意的是存在两个编码II类环丁烷嘧啶二聚体DNA光解酶的ORF。这两个ORF仅共享45%的氨基酸同一性，并且具有不同的启动子基序。还鉴定出另外22个独特的杆状病毒基因，包括一个编码新型推定的环指蛋白的基因，该蛋白在痘病毒中可能有同源物。
