bmp2a和bmp2b在斑马鱼晚期中鳍发育中的表达。

Expression of bmp2a and bmp2b in late-stage zebrafish median fin development.

作者信息

Crotwell Patricia L, Sommervold Amber R, Mabee Paula M

机构信息

Department of Biology, University of South Dakota, 414 East Clark Street, CL-191, Vermillion, SD 57069, USA.

出版信息

Gene Expr Patterns. 2004 Dec;5(2):291-6. doi: 10.1016/j.modgep.2004.07.001.

DOI:10.1016/j.modgep.2004.07.001

PMID:15567728

Abstract

Zebrafish bmp2a and bmp2b mRNA expression in the developing median fins (caudal, anal, and dorsal) of late-stage larvae (>5 days post-fertilization) was analyzed by reverse transcriptase-PCR (RT-PCR) and in situ hybridization. bmp2a is expressed in developing fin rays, while bmp2b is expressed in developing fin rays, hypertrophic chondrocytes, and in the zone of segmentation (ZS) in developing anal and dorsal fin radials. This latter pattern of bmp2b expression in the ZS mirrors tetrapod bmp2 expression in developing joints. Additionally, both genes are expressed in neural and hemal arches and spines. bmp2a is strongly expressed in the lens; lens bmp2b expression is detected only weakly via RT-PCR.

摘要

通过逆转录聚合酶链反应（RT-PCR）和原位杂交技术，分析了斑马鱼bmp2a和bmp2b mRNA在晚期幼虫（受精后>5天）发育中的中鳍（尾鳍、臀鳍和背鳍）中的表达情况。bmp2a在发育中的鳍条中表达，而bmp2b在发育中的鳍条、肥大软骨细胞以及发育中的臀鳍和背鳍桡骨的分割区（ZS）中表达。bmp2b在ZS中的这种表达模式与四足动物bmp2在发育关节中的表达相似。此外，这两个基因都在神经弓、血管弓和脊椎中表达。bmp2a在晶状体中强烈表达；通过RT-PCR仅微弱检测到晶状体bmp2b的表达。