斑马鱼 bmp2a 和 bmp2b 基因的功能分化。

Functional differentiation of bmp2a and bmp2b genes in zebrafish.

机构信息

College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.

出版信息

Gene Expr Patterns. 2022 Dec;46:119288. doi: 10.1016/j.gep.2022.119288. Epub 2022 Nov 2.

DOI:10.1016/j.gep.2022.119288

PMID:36332886

Abstract

Bone morphogenetic protein 2 plays an important role in the regulation of osteoblast proliferation and differentiation. Phylogenetic analysis showed that the bmp2 ortholog evolved from the same ancestral gene family in vertebrates and was duplicated in teleost, which were named bmp2a and bmp2b. The results of whole-mount in situ hybridization showed that the expression locations of bmp2a and bmp2b in zebrafish were different in different periods (24 hpf, 48 hpf, 72 hpf), which revealed potential functional differentiation between bmp2a and bmp2b. Phenotypic analysis showed that bmp2a mutations caused partial rib and vertebral deformities in zebrafish, while bmp2b embryos died massively after 12 hpf due to abnormal somite formation. We further explored the expression pattern changes of genes (bmp2a, bmp2b, smad1, fgf4, runx2b, alp) related to skeletal development at different developmental stages (20 dpf, 60 dpf, 90 dpf) in wild-type and bmp2a zebrafish. The results showed that the expression of runx2b in bmp2a was significantly downregulated at three stages and the expression of other genes were significantly downregulated at 90 dpf compared with wild-type zebrafish. The study revealed functional differentiation of bmp2a and bmp2b in zebrafish embryonic and skeletal development.

摘要

骨形态发生蛋白 2 在调节成骨细胞增殖和分化中发挥重要作用。系统发育分析表明，bmp2 直系同源物是从脊椎动物的同一祖先基因家族进化而来的，并在硬骨鱼中发生了重复，分别命名为 bmp2a 和 bmp2b。整体原位杂交的结果表明，bmp2a 和 bmp2b 在斑马鱼中的表达位置在不同时期（24 hpf、48 hpf、72 hpf）存在差异，这揭示了 bmp2a 和 bmp2b 之间可能存在功能分化。表型分析表明，bmp2a 突变导致斑马鱼部分肋骨和脊椎畸形，而 bmp2b 胚胎在 12 hpf 后由于体节形成异常而大量死亡。我们进一步探讨了不同发育阶段（20 dpf、60 dpf、90 dpf）野生型和 bmp2a 斑马鱼中与骨骼发育相关的基因（bmp2a、bmp2b、smad1、fgf4、runx2b、alp）的表达模式变化。结果表明，bmp2a 中 runx2b 的表达在三个阶段均显著下调，与野生型斑马鱼相比，其他基因在 90 dpf 的表达也显著下调。该研究揭示了 bmp2a 和 bmp2b 在斑马鱼胚胎和骨骼发育中的功能分化。