Yang Zhan-qing, Yu Shou-yi, Nie Jun, Chen Qing, Li Zhi-feng, Liu Yun-xi, Zhang Jin-lan, Xu Jian-jiang, Yu Xiao-ming, Bu Xiu-ping, Su Jing-jing, Zhang Yun, Tao Kai-hua
Department of Epidemiology, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Nov;24(11):1283-6.
To investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (HFRSV) infection in domestic pigs in Shandong province, and study the role of domestic pigs in the prevalence of HFRS.
Epidemiological investigation was performed in 4 cities of Shandong province. Reversed passive hemagglutination assay (RPHA), reversed passive hemagglutination inhibition (RPHI), HPR-SPA, immunofluorescent antibody (IFA), and reverse transcriptional PCR (RT-PCR) were used to detect antigen and antibody of HFRSV.
HFRSV antigen and antibody were detected in the heart, liver, lung, spleen, kidney, blood, urine, and stool of domestic pigs as well as in the sewage of the pigpen facilities. The positivity rate of HFRSV antigen ranged from 3.33% to 5.00% in the organ of pigs, and HFRSV positivity rate in the blood, urine, stool, and sewage was 3.67%, 7.04%, 2.51%, and 5.56%, respectively, with a total serum antibody positivity rate of 1.96%. The virus was isolated from the HFRSV antigen-positive samples, and could infect many organs after artificial infection of the pigs. HFRSV antigen can be detected in suckling rat brain and histopathological examination suggested transient pathological changes in such organs as the liver, lung and kidney. HFRSV may proliferate in pigs and was discharged through multiple routes.
Domestic pigs can be the host of HFRSV for the viral transmission.
探讨山东省家猪感染肾综合征出血热病毒（HFRSV）的流行病学意义，研究家猪在肾综合征出血热流行中的作用。
在山东省4个市进行流行病学调查。采用反向被动血凝试验（RPHA）、反向被动血凝抑制试验（RPHI）、HPR-SPA、免疫荧光抗体法（IFA）及逆转录聚合酶链反应（RT-PCR）检测HFRSV抗原和抗体。
在猪的心、肝、肺、脾、肾、血液、尿液、粪便以及猪圈设施污水中均检测到HFRSV抗原和抗体。猪器官中HFRSV抗原阳性率为3.33%～5.00%，血液、尿液、粪便及污水中HFRSV阳性率分别为3.67%、7.04%、2.51%和5.56%，血清抗体总阳性率为1.96%。从HFRSV抗原阳性样本中分离出病毒，人工感染猪后可感染多个器官。在乳鼠脑中可检测到HFRSV抗原，组织病理学检查提示肝、肺、肾等器官出现短暂病理变化。HFRSV可能在猪体内增殖并经多种途径排出。
家猪可作为HFRSV的宿主参与病毒传播。
