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一种用于检测不同家畜和野生动物中GII.6诺如病毒特异性IgG的新型荧光素酶免疫吸附测定法的开发与评估

Development and evaluation of a new luciferase immunosorbent assay to detect GII.6 norovirus-specific IgG in different domestic and wild animals.

作者信息

Liang Zhiyan, Zhang Minyi, Wang Yu, Koroma Mark Momoh, Yu Jingrong, Zhou Feiyuan, Jing Duona, Li Jiaheng, Tang Shixing, Chen Qing, Dai Ying-Chun

机构信息

Department of Epidemiology, School of Public Health, Southern Medical University (Guangdong Provincial Key Laboratory of Tropical Disease Research), Guangzhou, Guangdong, China.

出版信息

Front Microbiol. 2023 Jul 20;14:1213007. doi: 10.3389/fmicb.2023.1213007. eCollection 2023.

DOI:10.3389/fmicb.2023.1213007
PMID:37547694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10399220/
Abstract

Noroviruses (NoVs) are the leading viral pathogens globally causing acute gastroenteritis (AGE) in humans, posing a significant global health threat and economic burden. Recent investigations revealed that human NoVs had been detected in different animals, which raises concerns about whether NoVs are potential zoonotic diseases. This study developed a novel luciferase immunosorbent assay (LISA) to detect GII.6 NoV IgG based on P protein of VP1. The LISA showed high specificity (99.20%) and sensitivity (92.00%) with 4-16 times more sensitivity compared with an ELISA. NoV-LISA was reproducible with human serum regarding the inter- and intra-assay coefficient of variance values. Potential cross-reactivity was also evaluated using mice serum immunized by other antigens, which showed that NoV-LISA could differentiate GII.6 NoV from rotavirus and various genotypes of NoV. Specific GII.6 NoV IgG was widely detected in different domestic and wild animals, including dogs, pigs, bats, rats, and home shrews, with various IgG-positive rates ranging from 2.5 to 74.4%. In conclusion, our newly developed NoV-LISA assay is suitable for NoV-specific IgG detection in humans and animals. The wide distribution of IgG antibodies against human NoV indicates potential zoonotic transmission between humans and animals.

摘要

诺如病毒（NoVs）是全球范围内导致人类急性胃肠炎（AGE）的主要病毒病原体，对全球健康构成重大威胁并造成经济负担。最近的调查显示，在不同动物中检测到了人类诺如病毒，这引发了人们对诺如病毒是否为潜在人畜共患病的担忧。本研究基于VP1的P蛋白开发了一种新型的荧光素酶免疫吸附测定法（LISA）来检测GII.6型诺如病毒IgG。该LISA显示出高特异性（99.20%）和敏感性（92.00%），与酶联免疫吸附测定法（ELISA）相比，敏感性高4至16倍。就批间和批内变异系数值而言，诺如病毒-LISA在人血清中具有可重复性。还使用由其他抗原免疫的小鼠血清评估了潜在的交叉反应性，结果表明诺如病毒-LISA可以区分GII.6型诺如病毒与轮状病毒以及不同基因型的诺如病毒。在不同的家畜和野生动物中广泛检测到了特异性GII.6型诺如病毒IgG，包括狗、猪、蝙蝠、大鼠和家鼩，各种IgG阳性率在2.5%至74.4%之间。总之，我们新开发的诺如病毒-LISA测定法适用于人和动物中诺如病毒特异性IgG的检测。针对人类诺如病毒的IgG抗体的广泛分布表明人和动物之间存在潜在的人畜共患传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/087ce46361f9/fmicb-14-1213007-g0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/c14fd8defc96/fmicb-14-1213007-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/087ce46361f9/fmicb-14-1213007-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/dac7e6197426/fmicb-14-1213007-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/65638d777df7/fmicb-14-1213007-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/a76830ed4908/fmicb-14-1213007-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/9bdf3b609d61/fmicb-14-1213007-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/3add8119fdac/fmicb-14-1213007-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/c14fd8defc96/fmicb-14-1213007-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c796/10399220/087ce46361f9/fmicb-14-1213007-g0007.jpg

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