Pinheiro José Paulo, Bosker Wouter
Laboratory of Physical Chemistry and Colloid Science, Wageningen University and Research Center, Dreijenplein 6, 6703 HB Wageningen, The Netherlands.
Anal Bioanal Chem. 2004 Dec;380(7-8):964-8. doi: 10.1007/s00216-004-2843-3. Epub 2004 Nov 26.
A recently developed process for coating a glass surface with polystyrene (PS) film, by use of a simple chemical process has been used to reduce trace metal adsorption by cell components. The glass coating is a two-step procedure consisting of covalent attachment of vinyl-terminated PS to Si atoms on the glass surface then adsorption of PS from solution to create a stable PS film. To assess the quality of the coating we used anodic stripping voltammetry to study the adsorption of lead and cadmium ions in coated and untreated glass cells. In both short and long-term (24 h) experiments we observed that the amount of metal adsorbed was considerably less for the PS film-coated glass cell than for the uncoated cell. Further experiments showed that metal desorption is faster and metal contamination after cleaning is significantly lower for the coated cells. The PS film was, moreover, stable over a period of 6 months within the pH range 3.5-9.
最近开发的一种通过简单化学工艺在玻璃表面涂覆聚苯乙烯（PS）薄膜的方法，已被用于减少细胞成分对痕量金属的吸附。玻璃涂层是一个两步过程，包括将乙烯基封端的PS共价连接到玻璃表面的Si原子上，然后从溶液中吸附PS以形成稳定的PS薄膜。为了评估涂层的质量，我们使用阳极溶出伏安法研究了涂覆和未处理的玻璃细胞中铅和镉离子的吸附情况。在短期和长期（24小时）实验中，我们都观察到，PS薄膜涂覆的玻璃细胞吸附的金属量比未涂覆的细胞少得多。进一步的实验表明，涂覆细胞的金属解吸更快，清洗后的金属污染明显更低。此外，PS薄膜在3.5-9的pH范围内6个月内保持稳定。
