环磷酸腺苷（cAMP）对人早期卵巢卵泡的体外作用
In vitro effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles.
作者信息
Zhang Pu, Louhio Henna, Tuuri Timo, Sjöberg Jari, Hreinsson Julius, Telfer Evelyn E, Hovatta Outi
机构信息
Department of Clinical Science, Division of Obstetrics and Gynecology, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden.
出版信息
J Assist Reprod Genet. 2004 Aug;21(8):301-6. doi: 10.1023/b:jarg.0000043704.10845.87.
PURPOSE
To test the effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period.
METHODS
Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples.
RESULTS
On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%).
CONCLUSION
CyclicAMP promoted folliculogenesis and follicle survival during 14-21 days' culture of human ovarian cortical slices.
目的
检测环磷腺苷（cAMP）在延长培养期对人早期卵巢卵泡的作用。
方法
将16例接受妇科腹腔镜检查妇女捐赠的卵巢活检组织切成薄片，分别在存在和不存在0.5 mM 8-溴环磷腺苷（8-bromo-cAMP）的情况下平行培养1、2或3周。从所有样本的组织切片记录卵泡的发育阶段、大小和活力。
结果
与未用8-溴环磷腺苷培养的组织相比，在第14天，用8-溴环磷腺苷培养的皮质切片显示次级卵泡比例显著更高（50.0%对20.0%），原始卵泡比例更低（9.7%对26.7%）。在第21天，接受8-溴环磷腺苷处理的皮质切片中存活卵泡的比例显著高于未接受8-溴环磷腺苷处理的切片（79.6%对55.2%）。
结论
在人卵巢皮质切片14至21天的培养过程中，环磷腺苷促进卵泡发生和卵泡存活。
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