Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva and Sackler Faculty of Medicine,Tel Aviv University,Tel Aviv, Israel.
J Assist Reprod Genet. 2011 Sep;28(9):761-9. doi: 10.1007/s10815-011-9605-x. Epub 2011 Jul 23.
To compare macroporous alginate scaffolds with Matrigel for culturing frozen-thawed human primordial follicles in organ culture.
Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E(2)) production.
The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate scaffold cultured samples. E(2) production was similar in both groups.
Three dimensional alginate scaffolds are a promising putative in vitro technology for developing human primordial follicles.
比较大孔海藻酸钠支架与 Matrigel 在人原始卵泡冻融后器官培养中的应用。
12 名女孩/妇女捐献卵巢组织。一份组织样本在解冻后立即固定（未培养样本）。切片分别在 Matrigel 或海藻酸钠支架上培养 2 周，使用无血清培养基。生长评估包括卵泡计数和分类、免疫组织化学和 17β-雌二醇（E2）的产生。
与 Matrigel 相比，海藻酸钠支架培养的样本中发育卵泡的数量明显更高，同时海藻酸钠支架培养的样本中原始卵泡的数量比未培养的样本减少。培养 1 周后，Matrigel 培养的样本中闭锁卵泡的数量明显高于海藻酸钠支架培养的样本。两组的 E2 产生相似。
三维海藻酸钠支架是一种很有前途的开发人类原始卵泡的体外技术。
