Wang Zhijie, Song Yuxi, Sun Shuhan, Zhao Chang, Fu Shixin, Xia Cheng, Bai Yunlong
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Animals (Basel). 2022 Jan 24;12(3):285. doi: 10.3390/ani12030285.
Inactive ovaries (IO) accounts for 50% of ovarian disease in postpartum dairy cows, which seriously affects their reproductive efficiency. To investigate the metabolic changes in the serum and follicular fluid of dairy cows with IO during lactation, six estrus (E) cows and six IO cows at 50 to 55 days in milk were selected based on B ultrasonic detection and clinical manifestations. The differential metabolites in serum and follicular fluid between the E cows and IO cows were identified by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry, combined with multidimensional statistical methods. The results showed that dairy cows with IO were in a subclinical ketosis status where beta-hydroxybutyrate (BHB) exceeded 1.20 mmol/L, 14 differential metabolites in the serum of IO cows included 10 increased metabolites and 4 decreased metabolites, and 14 differential metabolites in the follicular fluid of IO cows included 8 increased metabolites and 6 decreased metabolites. These differential metabolites mainly involved nine metabolic pathways. The common enrichment pathway of different metabolites in serum and follicular fluid were glycerophospholipid metabolism and pentose and glucuronate interconversions. In conclusion, there were significant differences in the differential metabolites and enrichment pathways between serum and follicular fluid of IO cows, implying that there were complex changes in blood metabolism and local follicular metabolism of IO cows, whose interactions need further investigation.
静止性卵巢（IO）占产后奶牛卵巢疾病的50%，严重影响其繁殖效率。为了研究泌乳期患有IO的奶牛血清和卵泡液中的代谢变化，根据B超检测和临床表现，选择了6头处于发情期（E）的奶牛和6头产奶50至55天的IO奶牛。采用超高压液相色谱-四极杆飞行时间质谱联用多维统计方法，鉴定E奶牛和IO奶牛血清及卵泡液中的差异代谢物。结果表明，患有IO的奶牛处于亚临床酮病状态，其中β-羟基丁酸（BHB）超过1.20 mmol/L，IO奶牛血清中有14种差异代谢物，包括10种增加的代谢物和4种减少的代谢物，IO奶牛卵泡液中有14种差异代谢物，包括8种增加的代谢物和6种减少的代谢物。这些差异代谢物主要涉及9条代谢途径。血清和卵泡液中不同代谢物的共同富集途径是甘油磷脂代谢和戊糖与葡糖醛酸相互转化。总之，IO奶牛血清和卵泡液中的差异代谢物和富集途径存在显著差异，这意味着IO奶牛的血液代谢和局部卵泡代谢发生了复杂变化，其相互作用有待进一步研究。
