Gazit Y, Rossler Y, Wang S, Tang J, Lurie S
The Israel Cohen Institute for Biological Control, Citrus Marketing Board of Israel, P.O. Box 80, Bet-Dagan 50250, Israel.
J Econ Entomol. 2004 Oct;97(5):1540-6. doi: 10.1603/0022-0493-97.5.1540.
Two developmental stages of Ceratitis capitata (Wiedemann), 24-h-old eggs and third instars, 8 d after oviposition, were subjected to thermal exposures in a heating block system, at various temperatures of 46, 48, 50, and 52 degrees C to determine the thermal death kinetics of the insects. At these temperatures, 100% mortality was achieved by exposure of 300 C. capitata larvae for 60, 15, 4, and 1 min, respectively. The 0.5 order kinetic model had the best fit to the survival ratio for all the treatment temperatures, hence it was used for the prediction of the lethal times. The thermal death time (TDT) curves showed that the third instars were more heat-resistant than eggs, especially at the two low temperatures (46 and 48 degrees C). Under temperature-time combinations that did not result in complete kill, the thermal mortality for eggs was also significantly higher than that for third instars. The activation energy values calculated from the TDT curves were 490.6 and 551.9 kJ/mol, respectively, for thermal death of eggs and third instars.
将地中海实蝇（Ceratitis capitata (Wiedemann)）的两个发育阶段，即产卵24小时后的卵和产卵8天后的三龄幼虫，置于加热块系统中，在46、48、50和52摄氏度等不同温度下进行热暴露，以确定昆虫的热死亡动力学。在这些温度下，分别将300只地中海实蝇幼虫暴露60分钟、15分钟、4分钟和1分钟，可实现100%的死亡率。对于所有处理温度，0.5级动力学模型与存活率的拟合度最佳，因此用于预测致死时间。热死亡时间（TDT）曲线表明，三龄幼虫比卵更耐热，尤其是在两个低温（46和48摄氏度）下。在未导致完全死亡的温度-时间组合下，卵的热死亡率也显著高于三龄幼虫。根据TDT曲线计算出的卵和三龄幼虫热死亡的活化能值分别为490.6和551.9 kJ/mol。
