Picker S M, Stürner S S, Oustianskaja L, Gathof B S
Transfusion Medicine, University of Cologne, Cologne, Germany.
Vox Sang. 2004 Oct;87(3):173-81. doi: 10.1111/j.1423-0410.2004.00570.x.
Leucodepleted whole blood (LWB) is already widely used for autologous donations and could also be appropriate for certain instances of allogeneic transfusion provided that storage quality can be preserved at component-like levels.
Sixteen units of whole blood (WB), donated by healthy volunteers into CPDA-1 according to German guidelines, were leucofiltered prestorage and stored for up to 49 days. Unfiltered WB in CPDA-1 (UFWB, n=16) and filtered red blood cells in SAGM (RCC, n=14) served as controls. Several haematological, biochemical and coagulatory quality parameters were determined at designated time-points during storage.
Apart from significant differences (P<0.05) in haematocrit (56.2+/-3.6 vs. 37.9+/-3.9%), and in the plasma concentrations of free haemoglobin (93.1+/-37.8 vs. 57.8+/-24.3 g/dl), K+ (38.9+/-5.3 vs. 31.5+/-4.3 mm) and ATP (2.7+/-0.2 vs. 1.6+/-0.4 micromol/g haemoglobin), with higher levels detected in RCC, no remarkable differences (P>0.05) were observed regarding haemolysis (0.23+/-0.07% vs. 0.31+/-0.13) and pH value (6.63+/-0.03 vs. 6.62+/-0.02) between RCC and LWB at the end of storage. Lack of leucodepletion manifested in significantly (P<0.05) higher rates of haemolysis (0.44+/-0.21%), free haemoglobin (89.6+/-43.5 g/dl) and lower pH values (6.56+/-0.04). During 42 days of LWB storage, sufficient amounts (% of the initial mean value) were observed with stable (factor XI, 97.5+/-15.0) and labile (factor V, 92.9+/-18.0; factor VIII, 69.2+/-17.1) clotting factors and inhibitors (antithrombin III 88.9+/-9.5), without any signs of activated coagulation.
Our data indicate that the quality of LWB is comparable to that of components during 42 days of storage. Thus, LWB could be an interesting option for using to facilitate and economize the blood supply, especially for surgical or trauma patients.
白细胞滤除全血（LWB）已广泛用于自体献血，并且在能够将储存质量维持在类似成分血水平的情况下，也适用于某些异体输血情况。
16单位由健康志愿者按照德国指南采集至CPDA - 1中的全血（WB），在储存前进行白细胞过滤，并储存长达49天。CPDA - 1中的未过滤全血（UFWB，n = 16）和SAGM中的过滤红细胞（RCC，n = 14）作为对照。在储存期间的指定时间点测定了多个血液学、生化和凝血质量参数。
除了血细胞比容（56.2±3.6对37.9±3.9%）、游离血红蛋白血浆浓度（93.1±37.8对57.8±24.3 g/dl）、钾离子（38.9±5.3对31.5±4.3 mmol）和ATP（2.7±0.2对1.6±0.4 μmol/g血红蛋白）存在显著差异（P<0.05），RCC中这些指标水平更高外，在储存末期RCC和LWB之间关于溶血（0.23±0.07%对0.31±0.13）和pH值（6.63±0.03对6.62±0.02）未观察到显著差异（P>0.05）。未进行白细胞滤除表现为溶血率（0.44±0.21%）、游离血红蛋白（89.6±43.5 g/dl）显著更高（P<0.05）以及pH值更低（6.56±0.04）。在LWB储存42天期间，观察到稳定的（因子XI，97.5±15.0）和不稳定的（因子V，92.9±18.0；因子VIII，69.2±17.1）凝血因子及抑制剂（抗凝血酶III 88.9±9.5）有足够的量（占初始平均值的百分比），且无任何凝血激活迹象。
我们的数据表明，LWB在储存42天期间的质量与成分血相当。因此，LWB可能是一种有助于促进和节约血液供应的有趣选择，特别是对于外科手术或创伤患者。
