Gondo Shigeki, Yanase Toshihiko, Okabe Taijiro, Tanaka Tomoko, Morinaga Hidetaka, Nomura Masatoshi, Goto Kiminobu, Nawata Hajime
Department of Medicine and Bioregulatory Science, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan.
Genes Cells. 2004 Dec;9(12):1239-47. doi: 10.1111/j.1365-2443.2004.00801.x.
Bone marrow stem cells develop into haematopoietic and mesenchymal lineages, but have not been known to participate in steroidogenic cell production. Steroidogenic factor 1 (SF-1), also designated adrenal 4 binding protein (Ad4BP), is an essential orphan nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland development. In the present study, we revealed that the adenovirus-mediated forced expression of SF-1 can transform cultured primary long-term cultured bone marrow cells into steroidogenic cells, showing the de novo synthesis of multiple steroid hormones in response to adrenocorticotropic hormone (ACTH). This finding may provide an initial step in innovative autograft cell transfer therapy for steroid hormone deficiencies.
骨髓干细胞可发育成造血和间充质谱系，但此前并不认为其参与类固醇生成细胞的产生。类固醇生成因子1（SF-1），也被称为肾上腺4结合蛋白（Ad4BP），是类固醇生成以及肾上腺和性腺发育所必需的孤儿核受体。在本研究中，我们发现腺病毒介导的SF-1强制表达可将培养的原代长期培养骨髓细胞转化为类固醇生成细胞，这些细胞在促肾上腺皮质激素（ACTH）刺激下可从头合成多种类固醇激素。这一发现可能为类固醇激素缺乏的创新性自体移植细胞转移治疗提供了第一步。
