Oza V M, Jabbar A A, Hakobyan N, Kazarian T, Valentino L A
The Department of Pediatrics and the Rush Hemophilia and Thrombophilia Center, Rush University Medical Center and Rush Children's Hospital, Chicago, IL 60612, USA.
Haemophilia. 2004 Nov;10(6):732-4. doi: 10.1111/j.1365-2516.2004.01048.x.
Transfusion-transmitted virus (TTV) is a potential cause of post-transfusion hepatitis in patients with haemophilia. Plasma-derived clotting factor concentrates currently undergo processes that are effective in removal and inactivation of viruses such as HIV, hepatitis B and C; however, their effectiveness with respect to TTV is unknown. To determine if TTV DNA is present in plasma-derived concentrates of factor IX, we tested 14 lots of Mononine and compared the results with BeneFix. Nucleic acid isolation, followed by a two-round polymerase chain reaction (PCR) and agarose gel analysis indicated that all 17 lots were negative for TTV. Although TTV may be considered an emerging pathogen, no evidence of the virus was detected in the commercially available plasma-derived concentrate of FIX most commonly used to treat haemophilia B.
输血传播病毒（TTV）是血友病患者输血后肝炎的一个潜在病因。目前，血浆源性凝血因子浓缩物所经过的处理过程对去除和灭活诸如HIV、乙型和丙型肝炎病毒等病毒有效；然而，其对TTV的有效性尚不清楚。为了确定IX因子的血浆源性浓缩物中是否存在TTV DNA，我们检测了14批莫罗凝血因子，并将结果与贝赋进行比较。核酸分离，随后进行两轮聚合酶链反应（PCR）和琼脂糖凝胶分析表明，所有17批产品的TTV检测均为阴性。尽管TTV可能被视为一种新出现的病原体，但在最常用于治疗B型血友病的市售血浆源性FIX浓缩物中未检测到该病毒的证据。
