Takemoto Angélica Yukari, Okubo Patrícia, Saito Patricia Keiko, Yamakawa Roger Haruki, Watanabe Maria Angélica Ehara, Veríssimo da Silva Junior Waldir, Borelli Sueli Donizete, Bedendo João
Departamento de Enfermagem, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Braz J Microbiol. 2015 Mar 1;46(1):307-11. doi: 10.1590/S1517-838246120131195. eCollection 2015 Mar.
Patients who undergo dialysis treatment or a renal transplant have a high risk of blood-borne viral infections, including the Torque teno virus (TTV). This study identified the presence of TTV and its genome groups in blood samples from 118 patients in dialysis and 50 renal-transplant recipients. The research was conducted in a hospital in the city of Maringá, state of Paraná. The viral DNA, obtained from whole blood, was identified by using two nested Polymerase Chain Reactions (PCR). The frequencies of TTV were 17% and 36% in dialysis patients using the methodology proposed by Nishizawa et al . (1997) and Devalle and Niel (2004) , respectively, and 10% and 54% among renal-transplant patients. There was no statistically significant association between the frequency of the pathogen and the variables: gender, time in dialysis, time since transplant, blood transfusions, and the concomitant presence of hepatitis B, for either the dialysis patients or the renal-transplant recipients. Among dialysis patients and renal-transplant recipients, genogroup 5 was predominant (48% and 66% respectively), followed by genogroup 4 (37% and 48%) and genogroup 1 (23% and 25%). Genogroup 2 was present in both groups of patients. Some patients had several genogroups, but 46% of the dialysis patients and 51% of the renal-transplant recipients had only a single genogroup. This study showed a high prevalence of TTV in dialysis patients and renal-transplant recipients.
接受透析治疗或肾移植的患者有感染血源性病毒的高风险，包括Torque teno病毒（TTV）。本研究在118例透析患者和50例肾移植受者的血样中鉴定了TTV及其基因组群的存在。该研究在巴拉那州马林加市的一家医院进行。通过使用两种巢式聚合酶链反应（PCR）从全血中获得病毒DNA并进行鉴定。采用Nishizawa等人（1997年）和Devalle与Niel（2004年）提出的方法，透析患者中TTV的检出率分别为17%和36%，肾移植患者中为10%和54%。对于透析患者或肾移植受者，病原体的检出率与以下变量之间无统计学显著关联：性别、透析时间、移植后时间、输血以及乙型肝炎的合并感染情况。在透析患者和肾移植受者中，基因组群5占主导（分别为48%和66%），其次是基因组群4（37%和48%）和基因组群1（23%和25%）。两组患者中均存在基因组群2。一些患者有多个基因组群，但46%的透析患者和51%的肾移植受者仅有一个基因组群。本研究表明透析患者和肾移植受者中TTV的患病率很高。
