Dong G, Wu Z, Wang Q, Zhou Y
Department of Periodontology, College of Stomatology, Fourth Military Medical University.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2001 Jun;19(3):146-8.
The aim of this study is to evaluate the biological effects of insulin and/or transforming growth factor (TGF-beta) on alkaline phosphatase (ALPase) activity and protein synthesis of periodontal ligament (PDL) cells in vitro.
The fifth passage of PDL cells were cultured in a 5% humid CO2 environment at 37 degrees C by using Dulbecco minimum essential medium (DMEM) supplemented with 10% fetal bovine serum (FBS) for 24 hours, and then insulin, TGF-beta, and insulin and TGF-beta were added respectively in a medium of 1% FBS. After 5 days, their ALPase activities were measured by using a kinetic method, and the total protein content was measured by using Coomassie brilliant blue staining.
Both insulin and TGF-beta significantly increased the ALPase activity and the total protein content of PDL cells at the concentrations from 1.0 to 100 U/L, and of 0.1-100 micrograms/L. The most optimal concentrations of insulin and TGF-beta were respectively 10 U/L and 1 microgram/L. The synergistic effect of insulin (10 U/L) and TGF-beta (1 microgram/L) was observed (P < 0.01).
The results suggest that insulin and/or TGF-beta may promote the differentiation and protein synthesis of PDL cells. The combination of them may be valuable in the regeneration of periodontal tissue.
本研究旨在评估胰岛素和/或转化生长因子（TGF-β）对体外培养的牙周膜（PDL）细胞碱性磷酸酶（ALPase）活性和蛋白质合成的生物学效应。
将第5代PDL细胞在含10%胎牛血清（FBS）的杜尔贝科改良 Eagle 培养基（DMEM）中，于37℃、5%湿度的二氧化碳环境下培养24小时，然后分别在含1% FBS的培养基中添加胰岛素、TGF-β、胰岛素和TGF-β。5天后，采用动力学方法测定其ALPase活性，用考马斯亮蓝染色法测定总蛋白含量。
胰岛素和TGF-β在浓度为1.0至100 U/L以及0.1 - 100μg/L时，均显著提高了PDL细胞的ALPase活性和总蛋白含量。胰岛素和TGF-β的最适浓度分别为10 U/L和1μg/L。观察到胰岛素（10 U/L）和TGF-β（1μg/L）具有协同作用（P < 0.01）。
结果表明胰岛素和/或TGF-β可能促进PDL细胞的分化和蛋白质合成。它们的联合应用可能对牙周组织再生具有重要价值。
