黄热病/圣路易斯脑炎嵌合病毒的构建及其作为诊断工具的应用。
Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool.
作者信息
Pugachev Konstantin V, Guirakhoo Farshad, Mitchell Fred, Ocran Simeon W, Parsons Megan, Johnson Barbara W, Kosoy Olga L, Lanciotti Robert S, Roehrig John T, Trent Dennis W, Monath Thomas P
机构信息
Acambis Inc., 38 Sidney Street, Cambridge, MA 02139, USA.
出版信息
Am J Trop Med Hyg. 2004 Nov;71(5):639-45.
St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and cocirculate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.
圣路易斯脑炎（SLE）病毒和西尼罗河（WN）病毒在基因上密切相关，且在美国共同传播。病毒中和试验为这些病毒感染的血清学诊断提供了最具特异性的方法。然而，使用野生型SLE和WN病毒株进行实验室检测受到生物安全防护要求的限制。我们构建了两种高度减毒的黄热病（YF）病毒嵌合体，它们含有来自强毒株MSI - 7（在美国分离）或自然减毒株CorAn9124（阿根廷）SLE毒株的前膜 - 包膜（prM - E）蛋白基因。在使用患者血清进行的病毒中和试验中，YF/SLE（CorAn版本）病毒和先前构建的YF/WN嵌合体被证明能够特异性地区分确诊的人类SLE和WN病例。这些嵌合体有潜力用作针对SLE和WN的诊断试剂和疫苗。
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