Hattori J, Rutledge R, Labbé H, Brown D, Sunohara G, Miki B
Plant Research Centre, Agriculture Canada, Ottawa.
Mol Gen Genet. 1992 Mar;232(2):167-73. doi: 10.1007/BF00279993.
The acetohydroxyacid synthase (AHAS) gene from the Arabidopsis thaliana mutant line GH90 carrying the imidazolinone resistance allele imr1 was cloned. Expression of the AHAS gene under the control of the CaMV 35S promoter in transgenic tobacco resulted in selective imidazolinone resistance, confirming that the single base-pair change found near the 3' end of the coding region of this gene is responsible for imidazolinone resistance. A chimeric AHAS gene containing both the imr1 mutation and the csr1 mutation, responsible for selective resistance to sulfonylurea herbicides, was constructed. It conferred on transgenic tobacco plants resistance to both sulfonylurea and imidazolinone herbicides. The data illustrate that a multiple-resistance phenotype can be achieved in an AHAS gene through combinations of separate mutations, each of which individually confers resistance to only one class of herbicides.
克隆了来自携带咪唑啉酮抗性等位基因imr1的拟南芥突变株系GH90的乙酰羟酸合酶（AHAS）基因。该AHAS基因在CaMV 35S启动子控制下在转基因烟草中表达，产生了对咪唑啉酮的选择性抗性，证实了在该基因编码区3'端附近发现的单碱基对变化导致了对咪唑啉酮的抗性。构建了一个嵌合AHAS基因，它同时包含负责对磺酰脲类除草剂产生选择性抗性的imr1突变和csr1突变。它赋予转基因烟草植株对磺酰脲类和咪唑啉酮类除草剂的抗性。这些数据表明，通过单独突变的组合可以在一个AHAS基因中实现多重抗性表型，每个单独的突变仅赋予对一类除草剂的抗性。
