Department of Horticultural Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Plant Physiol. 1990 Nov;94(3):1111-5. doi: 10.1104/pp.94.3.1111.
Two cell lines of Datura innoxia resistant to two imidazolinone herbicides, imazapyr and imazaquin, were isolated from mutagenized, predominantly haploid cell suspension cultures. Both of the resistant variants were >1000-fold more resistant than the wild-type to the two imidazolinones. The variant resistant to imazapyr showed cross-resistance to imazaquin and vice versa, but no cross-resistance to a structurally different inhibitor, chlorsulfuron, a sulfonylurea herbicide, was observed. The target enzyme, acetolactate synthase, extracted from imidazolinone-resistant cell lines was not inhibited by imazapyr or imazaquin but was sensitive to chlorsulfuron indicating separable sites of action for these inhibitors. The variation in resistance and cross-resistance of chlorsulfuron-resistant (PK Saxena, J King [1988] Plant Physiol 86: 863-867) and imidazolinone-resistant cell lines of Datura innoxia demonstrates the possibility of separate mutations of acetolactate synthase gene resulting in specific phenotypes.
从诱变的、主要是单倍体细胞悬浮培养物中分离出对两种咪唑啉酮除草剂，即咪草烟和咪草烟，具有抗药性的 2 种颠茄细胞系。这两种抗性变体对这两种咪唑啉酮的抗性比野生型高 1000 倍以上。对咪草烟具有抗性的变体对咪草烟表现出交叉抗性，反之亦然，但对结构不同的抑制剂，磺酰脲类除草剂氯磺隆，没有观察到交叉抗性。从咪唑啉酮抗性细胞系中提取的靶酶，乙酰乳酸合酶，不受咪草烟或咪草烟抑制，但对氯磺隆敏感，表明这些抑制剂的作用部位可分离。对氯磺隆（PK Saxena，J King [1988] Plant Physiol 86: 863-867）和咪唑啉酮抗性颠茄细胞系的抗性和交叉抗性的变化表明，乙酰乳酸合酶基因可能发生了单独的突变，导致了特定的表型。
