Xie Wen-guang, Shao Ning-sheng, Ma Xiao-chang, Ding Qin-xue, Zhao Xin, Liu Nong-le, Wei Yu-shu, Wang Hui-xin, Chen Ke-ji
Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
Zhongguo Zhong Yao Za Zhi. 2004 Sep;29(9):877-82.
To study the serum proteome of rat endotoxemia treated by figwort root (FR).
The differences of serum proteome among rats treated with lipopolysaccharide (LPS), FR, LPS + FR and saline respectively were analyzed by two-dimensional electrophoresis (2DE) assay.
The volumes of sixteen serum proteins (xPr) in LPS induced-endotoxemia group were greatly changed compared with those of the control group. Among them, the volumes of xPr 16, 19 were significantly decreased, and the volumes of xPr 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly increased. When treated with FR, the volumes of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly decreased, and the volumes of xPr 8, 9, 11, 12, 23, 14 were back to normal level. Two factors statistic analysis showed that FR had interaction with LPS for xPr 1, 5, 8, 10, 11, 12, 18, 19, 20, 21, 22, and FR might be the functional antagonist of LPS. We also observed that the volumes of xPr 10, 13, 15, 20, 21, 22 were found to change significantly only in FR treated group but not in LPS treated group or control group. Interestingly, the volume of xPr 13, 20, 21, 22 were increased and the volume of xPr 10, 15 were decreased.
The molecular basis of therapeutic effect of FR on endotoxemia might be through the regulation of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23. We can use proteomic techniques to study the molecular mechanisms of diseases treated by functional Chinese herbs and the combination of different herbs is necessary for the treatment of endotoxemia, as FR can not regulated all the changed proteins induced by LPS.
研究玄参治疗大鼠内毒素血症的血清蛋白质组。
采用双向电泳（2DE）分析分别用脂多糖（LPS）、玄参、LPS + 玄参及生理盐水处理的大鼠血清蛋白质组的差异。
与对照组相比，LPS诱导的内毒素血症组中16种血清蛋白（xPr）的含量发生了显著变化。其中，xPr 16、19含量显著降低，xPr 1、2、3、4、5、6、7、8、9、11、12、14、18、23含量显著升高。用玄参处理后，xPr 1、6、7、8、9、11、12、14、18、23含量显著降低，且xPr 8、9、11、12、23、14含量恢复至正常水平。双因素统计分析表明，玄参与LPS对xPr 1、5、8、10、11、12、18、19、20、21、22存在相互作用，玄参可能是LPS的功能拮抗剂。我们还观察到，xPr 10、13、15、20、21、22的含量仅在玄参处理组中发生显著变化，而在LPS处理组和对照组中未发生变化。有趣的是，xPr 13、20、21、22含量升高，xPr 10、15含量降低。
玄参对内毒素血症治疗作用的分子基础可能是通过调节xPr 1、6、7、8、9、11、12、14、18、23实现的。我们可以利用蛋白质组学技术研究功能性中药治疗疾病的分子机制，且由于玄参不能调节LPS诱导的所有变化蛋白，因此治疗内毒素血症需要不同草药联合使用。
