在贝克曼库尔特IMMAGE系统上评估用于检测抗链球菌溶血素O和链球菌DNA酶B抗体的重组抗原，并与采用传统抗原的方法进行比较。

Evaluation of recombinant antigens for the measurement of antibodies to streptolysin O and streptococcal DNase B on the Beckman Coulter IMMAGE system and comparison with a method employing conventional antigens.

作者信息

Freyaldenhoven Bettina Sigrid, Moos Gabriele, Hinzmann Rolf Dieter, Lütticken Rudolf

机构信息

Institute of Medical Microbiology & National Reference Center for Streptococci, University Hospital, 52057 Aachen, Germany.

出版信息

Clin Lab. 2004;50(11-12):667-73.

PMID:15575308

Abstract

The performance of a newly devised method employing recombinant antigens for the determination of antibodies to streptolysin O and DNase B on the Beckman Coulter IMMAGE System was compared with a nephelometric assay using conventional antigens. Sera from 114 patients with or without evidence of streptococcal infection were analysed by the two techniques. The results obtained with the new method correlated well with those of the reference test. The sensitivity of a combination measurement of antibodies to streptolysin O and DNase B was higher than that obtained when measuring each parameter alone. Handling of the tests was easier on the Beckman Coulter IMMAGE System. In conclusion, the new test from Beckman Coulter for the measurement of antibodies to streptolysin O and DNase B performs well and equals measurements using conventional antigens on a nephelometer.

摘要

在贝克曼库尔特IMMAGE系统上，将一种使用重组抗原测定抗链球菌溶血素O和DNase B抗体的新方法的性能与使用传统抗原的比浊法进行了比较。采用这两种技术对114例有或无链球菌感染证据的患者血清进行了分析。新方法获得的结果与参考测试的结果相关性良好。抗链球菌溶血素O和DNase B抗体联合检测的灵敏度高于单独检测每个参数时的灵敏度。在贝克曼库尔特IMMAGE系统上进行检测更容易操作。总之，贝克曼库尔特公司用于测定抗链球菌溶血素O和DNase B抗体的新检测方法性能良好，与在比浊仪上使用传统抗原进行的检测相当。