Gella F J, Viver J, Canalias F, Beleta J, Gonzalez-Sastre F
Department de Bioquímica, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Spain.
Clin Chem. 1993 Jun;39(6):1007-11.
A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti-streptolysin O and the anti-DNase B tests.
已开发出一种用于测定人血清中抗NAD + 糖水解酶的比色法。该测定法包括通过抗体抑制NAD + 糖水解酶（EC 3.2.2.5），并使用NAD + 的酶放大系统测定未抑制的酶活性。该测定法易于实施，并且具有信号与抗体浓度直接相关的额外优点。对100份人血清获得的结果与通常用于获得链球菌感染或其并发症证据的其他测试（如抗链球菌溶血素O和抗DNA酶B测试）相比具有优势。
