Foltz Kathy R, Adams Nikki L, Runft Linda L
Department of Molecular, Cellular, and Developmental Biology and the Marine Science Institute, University of California, Santa Barbara, California 93106, USA.
Methods Cell Biol. 2004;74:39-74. doi: 10.1016/s0091-679x(04)74003-0.
The protocols outlined here hopefully will provide researchers with healthy, beautiful echinoderm oocytes, eggs, and embryos for experimental use. The large size of echinoderm oocytes and eggs, the ease with which they can be manipulated, and (in many species) their optical clarity, make them an ideal model system for studying not only the events specific to oocyte maturation and fertilization, but also for investigating more general questions regarding cell cycle regulation in an in vivo system. The quick rate at which development proceeds after fertilization to produce transparent embryos and larva makes the echinoderm an advantageous organism for studying deuterostome embryogenesis. Continued use of the echinoderms as model systems will undoubtedly uncover exciting answers to questions regarding fertilization, cell cycle regulation, morphogenesis, and how developmental events are controlled.
这里概述的实验方案有望为研究人员提供健康、优质的棘皮动物卵母细胞、卵子和胚胎用于实验。棘皮动物卵母细胞和卵子体积大，易于操作，并且（在许多物种中）具有光学透明性，这使其不仅成为研究卵母细胞成熟和受精特定事件的理想模型系统，还可用于研究体内系统中有关细胞周期调控的更普遍问题。受精后发育迅速产生透明胚胎和幼虫，这使得棘皮动物成为研究后口动物胚胎发生的优势生物。继续将棘皮动物用作模型系统无疑将揭示有关受精、细胞周期调控、形态发生以及发育事件如何被控制等问题的令人兴奋的答案。
