Oh Hyuncheol, Kang Dae-Gill, Kwon Ji-Woong, Kwon Tae-Oh, Lee Seung-Yeob, Lee Duck-Bae, Lee Ho-Sub
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Biol Pharm Bull. 2004 Dec;27(12):2035-7. doi: 10.1248/bpb.27.2035.
Bioassay-guided fractionation of the EtOAc-soluble extract of Sedum sarmentosum afforded a new flavonoid, quercetin-3-O-alpha-(6'''-caffeoylglucosyl-beta-1,2-rhamnoside) (1), along with four known flavonoids, quercetin 3-O-alpha-(6'''-p-coumaroylglucosyl-beta-1,2-rhamnoside) (2), isorhamnetin-3-beta-glucopyranoside (3), quercetin-3-beta-glucopyranoside (4), and kaempferol-3-alpha-arabinopyranoside (5). Purification of these compounds was conducted with the application of various chromatographic methods. Compounds 1-5 inhibited angiotensin I converting enzyme (ACE) activity in a concentration-dependent manner. Compounds 1-5 had 50% inhibitory concentration values of 158.9+/-11.1 microgM, 351.6+/-3.9 microgM, 408.9+/-4.6 microgM, 708.8+/-23.1 microgM, and 392.8+/-13.4 microgM.
对垂盆草乙酸乙酯可溶提取物进行生物活性导向分离，得到一种新的黄酮类化合物，槲皮素-3-O-α-(6'''-咖啡酰葡萄糖基-β-1,2-鼠李糖苷)(1)，以及四种已知的黄酮类化合物，槲皮素3-O-α-(6'''-对香豆酰葡萄糖基-β-1,2-鼠李糖苷)(2)、异鼠李素-3-β-葡萄糖苷(3)、槲皮素-3-β-葡萄糖苷(4)和山柰酚-3-α-阿拉伯糖苷(5)。采用多种色谱方法对这些化合物进行纯化。化合物1-5以浓度依赖的方式抑制血管紧张素I转换酶(ACE)的活性。化合物1-5的半数抑制浓度值分别为158.9±11.1 μM、351.6±3.9 μM、408.9±4.6 μM、708.8±23.1 μM和392.8±13.4 μM。
