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基于PCR和HinfI限制性内切酶的克鲁斯念珠菌分离株分型方法比较

Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates.

作者信息

Sancak Banu, Rex John H, Chen Enuo, Marr Kieren

机构信息

Department of Clinical Microbiology and Microbiology, Hacettepe University Medical School, Ankara 06100, Turkey.

出版信息

J Clin Microbiol. 2004 Dec;42(12):5889-91. doi: 10.1128/JCM.42.12.5889-5891.2004.

DOI:10.1128/JCM.42.12.5889-5891.2004
PMID:15583333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC535297/
Abstract

We compared HinfI restriction endonuclease-based analysis of genomic DNA with a PCR-based method for molecular typing of 90 Candida krusei isolates from 17 geographically related patients. Strain groupings by these methods were the same for 89 of 90 isolates. Ten of 17 patients were infected with related strains of C. krusei.

摘要

我们将基于HinfI限制性内切酶的基因组DNA分析方法与基于聚合酶链反应（PCR）的方法进行了比较，以对来自17例地理位置相关患者的90株克鲁斯念珠菌分离株进行分子分型。对于90株分离株中的89株，通过这些方法进行的菌株分组是相同的。17例患者中有10例感染了克鲁斯念珠菌的相关菌株。

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Candida krusei fungemia. An escalating serious infection in immunocompromised patients.克柔念珠菌血症。免疫功能低下患者中不断升级的严重感染。
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DNA fingerprinting patterns of Candida species using HinfI endonuclease.使用HinfI核酸内切酶对念珠菌属进行DNA指纹图谱分析。
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Typing Candida albicans oral isolates from human immunodeficiency virus-infected patients by multilocus enzyme electrophoresis and DNA fingerprinting.通过多位点酶电泳和DNA指纹图谱技术对人类免疫缺陷病毒感染患者的白色念珠菌口腔分离株进行分型。
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Increased incidence of fungemia caused by Candida krusei.克鲁斯念珠菌引起的真菌血症发病率增加。
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