Arif S, Barkham T, Power E G, Howell S A
Department of Microbial Diseases, St. John's Institute of Dermatology, London, United Kingdom.
J Clin Microbiol. 1996 Sep;34(9):2205-9. doi: 10.1128/jcm.34.9.2205-2209.1996.
A cluster of Candida glabrata isolates recovered from seven patients in an intensive care unit over a 10-week period were compared with a collection of isolates from six epidemiologically distinct outpatients and a reference strain by several DNA typing methods. Restriction enzyme analysis with HinII distinguished 13 strains from the 14 sources and was the method of choice. Pulsed-field gel electrophoresis and random amplification of polymorphic DNA both detected nine types from the 14 sources; however, the results of these two methods did not always correlate. These methods demonstrated that five of the seven patients had distinguishable strains and that cross-infection was unlikely.
在10周时间内从重症监护病房的7名患者身上分离出的一组光滑念珠菌菌株,与从6名流行病学上不同的门诊患者分离出的菌株集合以及一个参考菌株,通过几种DNA分型方法进行了比较。用HinII进行的限制性酶切分析从14个来源中区分出了13个菌株,是首选方法。脉冲场凝胶电泳和随机扩增多态性DNA均从14个来源中检测出9种类型;然而,这两种方法的结果并不总是相关。这些方法表明,7名患者中的5名携带可区分的菌株,交叉感染不太可能发生。