Linjawi S, Li T C, Tuckerman E M, Blakemore A I F, Laird S M
BMRC, Sheffield Hallam University, City Campus, Sheffield S1 1WB, UK.
J Reprod Immunol. 2004 Dec;64(1-2):145-55. doi: 10.1016/j.jri.2004.08.007.
Interleukin-11 (IL-11) is a member of the IL-6 family of cytokines. Previous studies have suggested that IL-11 may play a role in human endometrial function. In this study, we have used immunocytochemistry to compare endometrial IL-11Ralpha and IL-11 expression in precisely timed peri-implantation biopsies from 9 normal fertile women and 16 recurrent miscarriage (RM) women. Immunocytochemistry was semi-quantified by obtaining an H-score value, which showed increased expression of both IL-11 and IL-11Ralpha in epithelial cells compared to stromal cells in all biopsies. There was a significant (P<0.01) reduction in epithelial cell IL-11, but not stromal cell IL-11, expression in endometrium from RM women compared to normal fertile women. There were no significant differences in expression of IL-11Ralpha protein in both stromal and epithelial cells in endometrium from the two groups of women. This work shows the presence of IL-11 and IL-11Ralpha within the endometrium of RM women during the peri-implantation period. The decreased expression of IL-11 in epithelial endometrium in RM women suggests that this cytokine may play a role in preventing miscarriage.
白细胞介素-11（IL-11）是细胞因子IL-6家族的成员。先前的研究表明，IL-11可能在人类子宫内膜功能中发挥作用。在本研究中，我们使用免疫细胞化学方法，比较了9名正常生育女性和16名复发性流产（RM）女性在精确计时的着床期活检组织中子宫内膜IL-11Rα和IL-11的表达情况。通过获得H评分值对免疫细胞化学进行半定量分析，结果显示在所有活检组织中，上皮细胞中IL-11和IL-11Rα的表达均高于基质细胞。与正常生育女性相比，RM女性子宫内膜上皮细胞中IL-11的表达显著降低（P<0.01），但基质细胞中IL-11的表达无显著差异。两组女性子宫内膜基质细胞和上皮细胞中IL-11Rα蛋白的表达无显著差异。这项研究表明，在着床期，RM女性的子宫内膜中存在IL-11和IL-11Rα。RM女性子宫内膜上皮中IL-11表达降低，提示该细胞因子可能在预防流产中发挥作用。
