Hightower K, McCready J
Eye Research Institute, Oakland University, Rochester, Michigan.
Invest Ophthalmol Vis Sci. 1992 Apr;33(5):1783-7.
The purpose of this study was to investigate the physiological effects of irradiation in the spectral range 295-340 nm in cultured rabbit lenses. Ultraviolet B cataract was produced in lenses exposed to low levels of irradiation, 1-2 mW/cm2. Opacification was assessed by laser transmittance measurements. The changes observed during lens culture after a 1 hr dose (4 J/cm2) include a gradual increase in hydration, sodium concentration, and calcium levels. Loss in membrane voltage and a rise in 36Cl accumulation indicate that membrane permeability was increased. The cation pump was impaired within 20 hr of irradiation, as concluded by an observed fall in 22Na efflux. Availability of glucose for cation transport was diminished based on the reduced rate of uptake of tritiated 3-o-methylglucose in irradiated lenses, but this reduced accumulation was observed much later than was sodium elevation. Ionic imbalances and opacification required less than 1 d of culture for 4-12-wk-old lenses and required nearly 7 d of culture for 100-wk-old lenses.
本研究的目的是调查295 - 340纳米光谱范围内的辐射对培养的兔晶状体的生理影响。暴露于低水平辐射（1 - 2毫瓦/平方厘米）的晶状体产生了紫外线B性白内障。通过激光透射率测量评估晶状体混浊情况。在给予1小时剂量（4焦耳/平方厘米）后，在晶状体培养过程中观察到的变化包括水合作用、钠浓度和钙水平逐渐增加。膜电压降低和³⁶Cl积累增加表明膜通透性增加。如观察到的²²Na外流减少所示，阳离子泵在辐射后20小时内受损。基于辐照晶状体中³H标记的3 - o - 甲基葡萄糖摄取速率降低，阳离子转运所需的葡萄糖可用性降低，但这种积累减少比钠升高出现得晚得多。对于4 - 12周龄的晶状体，离子失衡和混浊在培养不到1天时出现，而对于100周龄的晶状体，则需要近7天的培养时间。
