Zhu Feng, Qiu Yong, Meng Kui, Cheng Jack-Chun-yiu
Spinal Service, Gulou Hospital, Nanjing University Medical school, Nanjing 210008, China.
Zhonghua Wai Ke Za Zhi. 2004 Oct 22;42(20):1221-4.
To compare the cell viability of chondrocytes between the anterior and the posterior spinal growth plates in adolescent idiopathic scoliosis (AIS) by proliferation and apoptosis labelling.
Seventeen AIS patients (4 male and 13 female, mean age 13.6 years old, ranged from 10 to 17 years old) were recruited in this study. Growth plates were harvested during anterior and posterior surgery. PCNA (proliferating cell nuclear antigen) and TUNEL (terminal deoxynucleotide transferase-mediated nick end labeling) were used for proliferation and apoptosis labeling on chondrocytes respectively.
In AIS, the distribution of the proliferating nests were denser and more parallel in anterior column than those in posterior under microscope observation. In the proliferative and hypertrophic zone the PCNA index and PCNA/TUNEL ratio were higher in the anterior column than those in the posterior column (P < 0.05 and P < 0.01 respectively). While in resting zone the differences were not so significant.
In adolescent idiopathic scoliosis the growth viability of chondrocytes is more vigorous in anterior spinal column than in the posterior column.
通过增殖和凋亡标记比较青少年特发性脊柱侧凸（AIS）患者脊柱生长板前后部软骨细胞的活力。
本研究招募了17例AIS患者（4例男性，13例女性，平均年龄13.6岁，年龄范围10至17岁）。在前后路手术中获取生长板。分别用增殖细胞核抗原（PCNA）和末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）对软骨细胞进行增殖和凋亡标记。
在AIS患者中，显微镜下观察发现前柱增殖巢的分布比后柱更密集且更平行。在增殖和肥大区，前柱的PCNA指数和PCNA/TUNEL比值高于后柱（分别为P < 0.05和P < 0.01）。而在静止区，差异不显著。
在青少年特发性脊柱侧凸中，脊柱前柱软骨细胞的生长活力比后柱更强。
