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低温胁迫下钼对冬小麦抗氧化防御系统和膜脂过氧化的影响

Effects of molybdenum on antioxidative defense system and membrane lipid peroxidation in winter wheat under low temperature stress.

作者信息

Sun Xue-Cheng, Hu Cheng-Xiao, Tan Qi-Ling

机构信息

Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr;32(2):175-82.

Abstract

Effects of molybdenum (Mo) on antioxidative defense system and membrane lipid peroxidation in leaves of winter wheat (Triticum aestivum H. var. Huamai 8) were investigated under low temperature stress. Results of experiments indicate that Mo application in winter wheat induced a dramatic decrease in electrolyte leakage and malondialdehyde content under low temperature stress. The activities of antioxidative enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), peroxidase (POX, EC 1.11.1.7) and catalase (CAT, EC 1.11.1.6) were increased by Mo application and the extents of increase at low temperature were higher than those at normal temperature. Mo application also caused a significant increase in the ascorbic acid (AsA) and proline contents both at normal and low temperature and following the low temperature stress the increases in ascorbic acid and proline contents in Mo-treated winter wheat were higher. There was no significant difference in carotenoid (CAR) content between with and without Mo treatment under normal temperature, while there was a significant increase in Mo treatment under low temperature stress. It could be speculated that Mo application enhanced cold resistance by increasing the capacity to scavenge active oxygen species and alleviating membrane damage in winter wheat under low temperature stress.

摘要

研究了低温胁迫下钼(Mo)对冬小麦(Triticum aestivum H. var. Huamai 8)叶片抗氧化防御系统和膜脂过氧化的影响。实验结果表明,在低温胁迫下,冬小麦施用钼可显著降低电解质渗漏率和丙二醛含量。施用钼可提高超氧化物歧化酶(SOD,EC 1.15.1.1)、过氧化物酶(POX,EC 1.11.1.7)和过氧化氢酶(CAT,EC 1.11.1.6)等抗氧化酶的活性,且低温下的提高幅度高于常温。施用钼还使常温及低温下的抗坏血酸(AsA)和脯氨酸含量显著增加,低温胁迫后,钼处理的冬小麦中抗坏血酸和脯氨酸含量的增加幅度更大。常温下,钼处理与未处理的类胡萝卜素(CAR)含量无显著差异,而在低温胁迫下,钼处理的类胡萝卜素含量显著增加。可以推测,施用钼通过提高冬小麦在低温胁迫下清除活性氧的能力和减轻膜损伤来增强其抗寒性。

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