Srivatsan M, Peretz B, Hallahan B, Talwalker R
Department of Physiology and Biophysics, University of Kentucky Medical Center, Lexington 40536-0084.
J Comp Physiol B. 1992;162(1):29-37. doi: 10.1007/BF00257933.
Hemolymph was examined in young (ca. 86 days old), mature (ca. 163 days old), and old (ca. 294 days old) Aplysia for age-related changes in constituent proteins. In young, mature, and old animals protein concentrations were 1.6 +/- 0.27, 1.41 +/- 0.53, and 1.45 +/- 0.43 mg.ml-1, respectively. The copper-containing respiratory protein, hemocyanin, measured by determining the copper concentration, was found to increase significantly from young (0.98 +/- 0.51 microgram.ml-1) to mature (2.02 +/- 0.95 micrograms.ml-1) Aplysia, with little change between mature and old (1.92 +/- 0.43 micrograms.ml-1) animals. These findings were consistent with the results obtained when hemocyanin was directly measured by spectrophotometric absorption at 340 nm. Acetylcholinesterase (AChE) was present in the hemolymph of Aplysia. Its activity was highest in mature animals (3121 +/- 1627 units.mg-1) and least in old animals (1463 +/- 599 units.mg-1). Young animals had intermediate levels (2080 +/- 762 units.mg-1). SDS-PAGE revealed a distinct pattern of protein bands for hemolymph from each age group; hemolymph from the young group contained six prominent protein bands with molecular weights (MW) from 13 to 300 kDa. Hemolymph of mature and old animals exhibited four and three prominent protein bands, respectively, with MW between 45 and 300 kDa. A prominent band at 97 kDa was present in samples from the mature group, but was faint in samples from the old group and absent in samples from the young group.(ABSTRACT TRUNCATED AT 250 WORDS)
研究人员检测了幼年（约86日龄）、成熟（约163日龄）和老年（约294日龄）海兔的血淋巴，以观察其组成蛋白随年龄的变化。幼年、成熟和老年动物的蛋白浓度分别为1.6±0.27、1.41±0.53和1.45±0.43mg/ml。通过测定铜浓度来检测含铜呼吸蛋白血蓝蛋白，结果发现从幼年海兔（0.98±0.51μg/ml）到成熟海兔（2.02±0.95μg/ml），血蓝蛋白显著增加，而成熟海兔和老年海兔（1.92±0.43μg/ml）之间变化不大。这些发现与通过在340nm处进行分光光度吸收直接测量血蓝蛋白所得到的结果一致。乙酰胆碱酯酶（AChE）存在于海兔的血淋巴中。其活性在成熟动物中最高（3121±1627单位/mg），在老年动物中最低（1463±599单位/mg）。幼年动物的活性处于中间水平（2080±762单位/mg）。SDS-PAGE显示每个年龄组血淋巴的蛋白条带模式不同；幼年组血淋巴包含六条显著的蛋白条带，分子量（MW）在13至300kDa之间。成熟和老年动物的血淋巴分别显示四条和三条显著的蛋白条带，MW在45至300kDa之间。成熟组样品中存在一条97kDa的显著条带，但在老年组样品中较淡，在幼年组样品中不存在。（摘要截取自250字）
