Wilson Gayle M, Fielding Andrew B, Simon Glenn C, Yu Xinzi, Andrews Paul D, Hames Rebecca S, Frey Andrew M, Peden Andrew A, Gould Gwyn W, Prekeris Rytis
Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Mol Biol Cell. 2005 Feb;16(2):849-60. doi: 10.1091/mbc.e04-10-0927. Epub 2004 Dec 15.
An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.
细胞分裂的一个不可或缺的部分是通过胞质分裂来分离子细胞。现在有充分的证据表明，胞质分裂的完成需要协调的膜运输，以便将新的膜输送到沟的顶端并完成脱离。在这里，我们研究了胞质分裂中的膜运输，并描述了几个新的观察结果。首先，我们表明，含有Rab11和FIP3的回收型内体在分裂沟附近积累，并且是胞质分裂成功完成所必需的。其次，我们证明Rab11-FIP3蛋白复合物密切参与内体向分裂沟的运输。值得注意的是，虽然FIP3向内体的募集依赖于Rab11，但我们发现FIP3向中体的靶向独立于Rab11。第三，我们表明Rab11-FIP3复合物是胞质分裂后期（可能是脱离阶段）所必需的。最后，我们证明FIP3的定位受到大量的空间和时间调控。这些数据首次对细胞分裂中的回收型内体进行了详细分析，并为向沟的膜运输提供了一个新模型。我们提出，动态的Rab11-FIP3相互作用在胞质分裂后期和脱离过程中控制回收型内体向沟的运输、靶向和融合。
