嗜酸性红假单胞菌7750菌株B800-820光捕获复合体的结晶

Crystallization of the B800-820 light-harvesting complex from Rhodopseudomonas acidophila strain 7750.

作者信息

Guthrie N, MacDermott G, Cogdell R J, Freer A A, Isaacs N W, Hawthornthwaite A M, Halloren E, Lindsay J G

机构信息

Department of Chemistry, University of Glasgow, Scotland.

出版信息

J Mol Biol. 1992 Mar 20;224(2):527-8. doi: 10.1016/0022-2836(92)91016-i.

DOI:10.1016/0022-2836(92)91016-i

PMID:1560469

Abstract

The B800-820 light-harvesting complex, an integral membrane protein, from Rhodopseudomonas acidophila strain 7750 has been crystallized. The tabular plates have a hexagonal unit cell of a = b = 121.8 A and c = 283.1 A and belong to the space group R32. X-ray diffraction data have been collected to 6 A resolution, using an area detector on a rotating anode source. The B800-820 light-harvesting complex is comprised of four low molecular weight apoproteins (B800-820 alpha 1, B800-820 alpha 2, B800-820 beta 1 and B800-820 beta 2). Polyacrylamide gel electrophoresis shows that the complex exists as an oligomeric assembly, with an apparent molecular weight of 92,000.

摘要

来自嗜酸红假单胞菌7750菌株的B800 - 820捕光复合体（一种整合膜蛋白）已被结晶。平板晶体具有六方晶胞，a = b = 121.8 Å，c = 283.1 Å，属于空间群R32。使用旋转阳极源上的面积探测器收集了分辨率为6 Å的X射线衍射数据。B800 - 820捕光复合体由四种低分子量脱辅基蛋白（B800 - 820α1、B800 - 820α2、B800 - 820β1和B800 - 820β2）组成。聚丙烯酰胺凝胶电泳表明该复合体以寡聚体形式存在，表观分子量为92,000。