嗜酸红假单胞菌10050菌株B800 - 850光捕获复合体两种晶型的结晶与表征

Crystallization and characterization of two crystal forms of the B800-850 light-harvesting complex from Rhodopseudomonas acidophila strain 10050.

作者信息

Papiz M Z, Hawthornthwaite A M, Cogdell R J, Woolley K J, Wightman P A, Ferguson L A, Lindsay J G

机构信息

Department of Botany, University of Glasgow, Scotland.

出版信息

J Mol Biol. 1989 Oct 20;209(4):833-5. doi: 10.1016/0022-2836(89)90612-8.

DOI:10.1016/0022-2836(89)90612-8

PMID:2685328

Abstract

Two different crystal forms of the B800-850-antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown. This complex is an integral membrane protein and is isolated as an oligomeric assembly with a molecular weight of approximately 84 kDa. This assembly contains six alpha/beta apoprotein pairs, 18 molecules of bacteriochlorophyll a and nine molecules of carotenoid. The first crystal form has dimensions unit cell a = b = 75.8 A, c = 97.5 A with the space group P4 and diffracts to a resolution of 12.0 A. The second crystal form is rhombohedral with dimensions unit cell a = 121.1 A, alpha = 60 degrees, space group R32 and diffracts to a resolution of 3.5 A. Native data have been processes in both cases, to an Rmerge value of 9.0 to 11.0%. The X-ray data suggest that the asymmetric unit, in both crystal forms, contains one 84 kDa antenna complex.

摘要

已培养出嗜酸红假单胞菌10050菌株B800 - 850天线复合物的两种不同晶体形式。该复合物是一种整合膜蛋白，以分子量约为84 kDa的寡聚体形式分离得到。该寡聚体包含六个α/β脱辅基蛋白对、18个细菌叶绿素a分子和九个类胡萝卜素分子。第一种晶体形式的晶胞尺寸为a = b = 75.8 Å，c = 97.5 Å，空间群为P4，衍射分辨率为12.0 Å。第二种晶体形式为菱面体，晶胞尺寸为a = 121.1 Å，α = 60°，空间群为R32，衍射分辨率为3.5 Å。两种情况下均已处理原生数据，合并R值为9.0%至11.0%。X射线数据表明，两种晶体形式的不对称单元均包含一个84 kDa的天线复合物。