Wang Haikuan, Lu Fuping, Sun Yafan, Du Lianxiang
Department of Food Science and Biology Engineering, Tianjin Science and Technology University, P.R. China.
Biotechnol Lett. 2004 Oct;26(20):1569-73. doi: 10.1023/B:BILE.0000045654.66689.b4.
The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae alpha-factor signal peptide. Both peptides efficiently directed the secretion of lignin peroxidase from the recombinant yeast cell. The extracellular lignin peroxidase activity in two recombinants was 932 U l(-1) and 1933 U l(-1). The purity of the recombinant product was confirmed by SDS-PAGE.
编码黄孢原毛平革菌木质素过氧化物酶的cDNA在甲醇毕赤酵母中，在醇氧化酶（AUG1）启动子的控制下表达，该启动子之后接黄孢原毛平革菌的木质素过氧化物酶前导肽或酿酒酵母α-因子信号肽。两种肽都有效地引导了重组酵母细胞中木质素过氧化物酶的分泌。两个重组体中的细胞外木质素过氧化物酶活性分别为932 U l(-1)和1933 U l(-1)。通过SDS-PAGE证实了重组产物的纯度。
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