毕赤酵母中里氏木霉内切 1,4-β-木聚糖酶 C 的异源表达。
Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris.
机构信息
Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Republic of Korea.
出版信息
J Biosci Bioeng. 2011 Jun;111(6):654-7. doi: 10.1016/j.jbiosc.2011.02.010. Epub 2011 Mar 9.
PMID:21393056
Abstract
The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).
摘要
从黄孢原毛平革菌中分离得到的内切 1,4-β-木聚糖酶 C 的 cDNA 在毕赤酵母中表达，受醇氧化酶 I 启动子的控制。无论是使用木聚糖酶 C 的固有启动肽还是酿酒酵母的α-因子信号肽，木聚糖酶 C 都能有效地分泌到培养基中，最高浓度可达 2500 U·l(-1)。
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