使用海藻糖改善大肠杆菌感受态细胞的长期冷冻保存
Improved long-term cryostorage of Escherichia coli competent cells using trehalose.
作者信息
Ahn Taeho, Kang Seong Su, Yun Chul-Ho
机构信息
Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
出版信息
Biotechnol Lett. 2004 Oct;26(20):1593-4. doi: 10.1023/B:BILE.0000045659.41621.de.
Abstract
Trehalose added to storage buffers for chemical-induced transformations at 200 mM increased the survival of Escherichia coli competent cells 1.3 approximately 4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.
摘要
在用于化学诱导转化的储存缓冲液中添加200 mM海藻糖，与二甲基亚砜或甘油相比，在180天的冷冻储存期间，可使大肠杆菌感受态细胞的存活率提高约1.3至4.8倍。
Zotero 插件