冠心病患者中组织因子途径抑制物（TFPI）、脂蛋白（a）[Lp（a）]及氧化型低密度脂蛋白抗体水平之间的关系。

The relationship of TFPI, Lp(a), and oxidized LDL antibody levels in patients with coronary artery disease.

作者信息

Bilgen Dilek, Sönmez Hüseyin, Ekmekçi Hakan, Ulutin Turgut, Oztürk Zeynep, Kökoğlu Emine, Bayram Ciğdem, Soner Ali, Domaniç Nergiz

机构信息

Department of Biochemistry, Cerrahpasa Medical Faculty, University of Istanbul, Turkey.

出版信息

Clin Biochem. 2005 Jan;38(1):92-6. doi: 10.1016/j.clinbiochem.2004.09.011.

DOI:10.1016/j.clinbiochem.2004.09.011

PMID:15607324

Abstract

OBJECTIVES

The aim of the present study was to determine and correlate tissue factor pathway inhibitor (TFPI), lipoprotein (a) (Lp(a)), oxidized low-density lipoprotein (LDL) antibody (oLAB), and thiobarbituric acid reactive substances (TBARS; as a marker of lipid peroxidation) levels in patients with coronary artery disease (CAD) and in a control group.

DESIGN AND METHODS

Peripheral blood samples from patients with coronary heart disease were provided by the Department of Cardiology. Serum oLAB, Lp(a), plasma total TFPI, and plasma-free TFPI levels were determined by ELISA. Serum TBARS levels were determined by a spectrophotometric method using thiobarbituric acid.

RESULTS

The CAD and the control group were matched for age and sex. Serum Lp(a), oLAB, and plasma total TFPI levels in patients with coronary heart disease were found to be significantly higher than in the control group (P < 0.001). But there was no difference in plasma-free TFPI levels between patients with CAD and the control group (P > 0.05). In patients with single (P < 0.05), double, and triple vessel (P < 0.01) disease, the mean serum Lp(a) levels were significantly higher than in the control group. On the other hand, in patients with single vessel disease (P < 0.05), double vessel disease (P < 0.05), and triple vessel disease (P < 0.001), plasma total TFPI levels were found to be significantly higher than in the control group. We also found a significant positive correlation (r = 0.28, P < 0.05) between serum Lp(a) and plasma total TFPI levels in CAD. In the patient group, TBARS, total cholesterol, triglyceride (TRG), and LDL cholesterol levels were found to be significantly higher than those in the control group. In addition, high-density lipoprotein (HDL) cholesterol levels were found to be significantly lower than the control group.

CONCLUSIONS

These results suggest that elevated plasma levels of total TFPI, Lp(a), and oLAB may be useful diagnostic and monitoring markers in patients with CAD.

摘要

目的

本研究旨在测定冠状动脉疾病（CAD）患者及对照组中组织因子途径抑制剂（TFPI）、脂蛋白（a）[Lp（a）]、氧化型低密度脂蛋白（LDL）抗体（oLAB）以及硫代巴比妥酸反应性物质（TBARS，作为脂质过氧化的标志物）的水平，并进行相关性分析。

设计与方法

冠心病患者的外周血样本由心脏病科提供。采用酶联免疫吸附测定法（ELISA）测定血清oLAB、Lp（a）、血浆总TFPI和游离TFPI水平。采用硫代巴比妥酸分光光度法测定血清TBARS水平。

结果

CAD组和对照组在年龄和性别上相匹配。发现冠心病患者的血清Lp（a）、oLAB和血浆总TFPI水平显著高于对照组（P < 0.001）。但CAD患者与对照组的游离TFPI水平无差异（P > 0.05）。在单支血管病变（P < 0.05）、双支血管病变和三支血管病变（P < 0.01）的患者中，平均血清Lp（a）水平显著高于对照组。另一方面，在单支血管病变（P < 0.05）、双支血管病变（P < 0.05）和三支血管病变（P < 0.001）的患者中，血浆总TFPI水平显著高于对照组。我们还发现CAD患者血清Lp（a）与血浆总TFPI水平之间存在显著正相关（r = 0.28，P < 0.05）。在患者组中，TBARS、总胆固醇、甘油三酯（TRG）和低密度脂蛋白胆固醇水平显著高于对照组。此外，高密度脂蛋白（HDL）胆固醇水平显著低于对照组。