卵巢功能不全中卵母细胞线粒体DNA含量低。
Low oocyte mitochondrial DNA content in ovarian insufficiency.
作者信息
May-Panloup P, Chrétien M F, Jacques C, Vasseur C, Malthièry Y, Reynier P
机构信息
Biologie de la Reproduction--Laboratoire FIV, Centre Hospitalier Universitaire d'Angers, 4, rue Larrey, F-49033 Angers cedex 01, France.
出版信息
Hum Reprod. 2005 Mar;20(3):593-7. doi: 10.1093/humrep/deh667. Epub 2004 Dec 17.
BACKGROUND
Mitochondrial biogenesis and bioenergetics play an important role in oocyte maturation and embryo development. We have investigated the relationship between defective mitochondrial biogenesis and the lack of oocyte maturity observed during IVF procedures with patients suffering from ovarian dystrophy and ovarian insufficiency.
METHODS
We used real-time quantitative PCR to quantify mitochondrial DNA (mtDNA) in 116 oocytes obtained from 47 women undergoing the ICSI procedure. We compared the mtDNA content of oocytes from women with a normal ovarian profile with that of oocytes from women with ovarian dystrophy and ovarian insufficiency.
RESULTS
We found an average of 256,000 +/- 213,000 mitochondrial genomes per cell. The mean mtDNA copy number was not significantly different in ovarian dystrophy compared with controls, but it was significantly lower in oocytes from women with ovarian insufficiency (100,000 +/- 99,000, P < 0.0001).
CONCLUSIONS
Our results suggest that low mtDNA content is associated with the impaired oocyte quality observed in ovarian insufficiency.
背景
线粒体生物合成和生物能量学在卵母细胞成熟和胚胎发育中起着重要作用。我们研究了线粒体生物合成缺陷与体外受精（IVF）过程中观察到的患有卵巢发育不良和卵巢功能不全患者卵母细胞成熟度缺乏之间的关系。
方法
我们使用实时定量聚合酶链反应（PCR）对从47名接受卵胞浆内单精子注射（ICSI）手术的女性获得的116个卵母细胞中的线粒体DNA（mtDNA）进行定量。我们比较了卵巢功能正常女性的卵母细胞与卵巢发育不良和卵巢功能不全女性的卵母细胞的mtDNA含量。
结果
我们发现每个细胞平均有256,000±213,000个线粒体基因组。与对照组相比，卵巢发育不良患者的平均mtDNA拷贝数无显著差异，但卵巢功能不全女性的卵母细胞中该数值显著较低（100,000±99,000，P<0.0001）。
结论
我们的结果表明，低mtDNA含量与卵巢功能不全中观察到的卵母细胞质量受损有关。
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