Yoshida A, Tachibana M, Ansai T, Takehara T
Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan.
Oral Microbiol Immunol. 2005 Feb;20(1):43-6. doi: 10.1111/j.1399-302X.2004.00179.x.
Prevotella species are a major component of the oral microflora and some have been implicated in various forms of periodontal disease. Despite the importance of understanding the prevalence of these organisms in the oral microflora, no rapid, simultaneous detection system for these species has been reported. This study developed a multiplex polymerase chain reaction assay for the simultaneous detection of four oral black-pigmented Prevotella species in various oral specimens. This assay will be useful for determining the prevalence of these organisms in the oral ecosystem. Furthermore, this assay system should prove a useful tool for analyzing the role of black-pigmented Prevotella species in the mouth.
普雷沃菌属是口腔微生物群的主要组成部分，其中一些与多种形式的牙周疾病有关。尽管了解这些微生物在口腔微生物群中的流行情况很重要，但尚未有针对这些菌种的快速、同步检测系统的报道。本研究开发了一种多重聚合酶链反应检测方法，用于同时检测各种口腔标本中的四种口腔产黑色素普雷沃菌。该检测方法将有助于确定这些微生物在口腔生态系统中的流行情况。此外，该检测系统应被证明是分析产黑色素普雷沃菌在口腔中作用的有用工具。
