Costa Marília Teixeira, Dorta Miriam Leandro, Ribeiro-Dias Fátima, Pimenta Fabiana Cristina
Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil.
Braz Dent J. 2012;23(5):555-8. doi: 10.1590/s0103-64402012000500014.
This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.
本研究采用多重聚合酶链反应（PCR）技术，调查有或无黑色外源性牙渍患者牙菌斑中黑色色素细菌变黑普氏菌和中间普氏菌、非黑色色素细菌放线菌属，尤其是致龋病原菌变形链球菌的存在情况。对有（n = 26）或无（n = 26）黑色牙渍患者的牙菌斑进行分析，使用针对16S核糖体RNA基因（变黑普氏菌、中间普氏菌、放线菌属）的双重PCR和针对变形链球菌的葡糖基转移酶-I基因进行检测。变黑普氏菌和变形链球菌是两组中最常检测到的细菌。检测频率最低的是中间普氏菌和放线菌属。黑色牙渍和健康牙齿表面牙菌斑的细菌组成相似，表明黑色牙渍中也存在致龋细菌。
