Riggio M P, Lennon A, Rolph H J, Hodge P J, Donaldson A, Maxwell A J, Bagg J
Infection and Immunity Research Group, University of Glasgow Dental School, Glasgow, UK.
Oral Dis. 2008 Apr;14(3):251-8. doi: 10.1111/j.1601-0825.2007.01371.x.
Compare the microbial profiles on the tongue dorsum in patients with halitosis and control subjects in a UK population using culture-independent techniques.
Halitosis patients were screened according to our recently developed recruitment protocol. Scrapings from the tongue dorsum were obtained for 12 control subjects and 20 halitosis patients. Bacteria were identified by PCR amplification, cloning and sequencing of 16S rRNA genes.
The predominant species found in the control samples were Lysobacter-type species, Streptococcus salivarius, Veillonella dispar, unidentified oral bacterium, Actinomyces odontolyticus, Atopobium parvulum and Veillonella atypica. In the halitosis samples, Lysobacter-type species, S. salivarius, Prevotella melaninogenica, unidentified oral bacterium, Prevotella veroralis and Prevotella pallens were the most commonly found species. For the control samples, 13-16 (4.7-5.8%) of 276 clones represented uncultured species, whereas in the halitosis samples, this proportion increased to 6.5-9.6% (36-53 of 553 clones). In the control samples, 22 (8.0%) of 276 clones represented potentially novel phylotypes, and in the halitosis samples, this figure was 39 (7.1%) of 553 clones.
The microflora associated with the tongue dorsum is complex in both the control and halitosis groups, but several key species predominate in both groups.
运用非培养技术比较英国人群中口臭患者与对照者舌背的微生物谱。
根据我们最近制定的招募方案筛选口臭患者。采集了12名对照者和20名口臭患者的舌背刮屑。通过对16S rRNA基因进行PCR扩增、克隆和测序来鉴定细菌。
在对照样本中发现的主要菌种为溶杆菌属菌种、唾液链球菌、殊异韦荣球菌、未鉴定的口腔细菌、解齿放线菌、微小阿托波菌和非典型韦荣球菌。在口臭样本中，最常见的菌种为溶杆菌属菌种、唾液链球菌、产黑素普雷沃菌、未鉴定的口腔细菌、口腔普雷沃菌和苍白普雷沃菌。对于对照样本而言，276个克隆中有13 - 16个（4.7 - 5.8%）代表未培养菌种，而在口臭样本中，这一比例增至6.5 - 9.6%（553个克隆中有36 - 53个）。在对照样本中，276个克隆中有22个（8.0%）代表潜在的新系统发育型，而在口臭样本中，这一数字为553个克隆中的39个（7.1%）。
对照组和口臭组中与舌背相关的微生物群均很复杂，但两组中均有几种关键菌种占主导地位。
