Nastke M-D, Herrgen L, Walter S, Wernet D, Rammensee H-G, Stevanović S
Department of Immunology, Institute for Cell Biology, Auf der Morgenstelle 15, 72076 Tübingen, Germany.
Cell Mol Life Sci. 2005 Jan;62(1):77-86. doi: 10.1007/s00018-004-4363-x.
Human cytomegalovirus (HCMV) infection or reactivation is a cause of morbidity and mortality in immunocompromised individuals. In immunocompetent individuals, in contrast, HCMV is successfully controlled by specific CD8 and CD4 T cells. Knowledge of CD8 and CD4 T cell epitopes from HCMV and their immunodominant features is crucial for the generation of epitope-specific T cells for adoptive immunotherapy and for the development of a peptide-based HCMV vaccine. Therefore, we investigated the natural frequencies of a large number of CD8 and CD4 T cell epitopes, including 10 novel ones. We determined several epitopes as immunodominant. Surprisingly, no clear hierarchies were found for CD8 T cell epitopes, indicating codominance. These results will be valuable for adoptive transfer strategies and support initiatives towards development of a peptide-based HCMV vaccine.
人巨细胞病毒（HCMV）感染或再激活是免疫功能低下个体发病和死亡的一个原因。相比之下，在免疫功能正常的个体中，HCMV可被特异性CD8和CD4 T细胞成功控制。了解HCMV的CD8和CD4 T细胞表位及其免疫显性特征对于生成用于过继免疫治疗的表位特异性T细胞以及开发基于肽的HCMV疫苗至关重要。因此，我们研究了大量CD8和CD4 T细胞表位的自然频率，其中包括10个新表位。我们确定了几个表位具有免疫显性。令人惊讶的是，未发现CD8 T细胞表位有明确的层次结构，表明存在共显性。这些结果对于过继转移策略将具有重要价值，并为基于肽的HCMV疫苗的开发提供支持。
