Chirhart Scott E, Honeycutt Rodney L, Greenbaum Ira F
Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA.
Mol Phylogenet Evol. 2005 Feb;34(2):408-15. doi: 10.1016/j.ympev.2004.10.018. Epub 2004 Dec 15.
Variation at 12 pure-repeat dinucleotide microsatellites from Peromyscus maniculatus was analyzed for samples of all species in the P. maniculatus species group and P. leucopus. Except for one locus (Pml08) that amplified a product only for P. maniculatus, these microsatellites yielded reliable estimates of variation across these species; per-locus polymorphism and allele-size distribution were not significantly different among or between any of the species sampled from mainland populations. Significantly lower levels of variation and the distribution of alleles in the two populations of the insular-endemic P. sejugis were consistent with the expectation of substantial founder effect and suggest a lack of recent gene flow between these populations. Phenetic analyses of genetic distances based on shared allele frequencies uniformly produced well-supported trees that were entirely concordant with the a priori corroborated relationships within the P. maniculatus species group; this result was not obtained with analyses of a genetic distance computed from differences in allele sizes. The microsatellite data do not support the hypothesis that P. sejugis should be considered conspecific with P. maniculatus but yield a strongly supported sister-group association between P. sejugis and P. keeni.
对鹿鼠（Peromyscus maniculatus）物种组中的所有物种以及白足鼠（P. leucopus）的样本，分析了12个纯重复二核苷酸微卫星的变异情况。除了一个位点（Pml08）仅对鹿鼠扩增出产物外，这些微卫星对这些物种的变异产生了可靠的估计；从大陆种群采样的任何物种之间或之内，每个位点的多态性和等位基因大小分布均无显著差异。岛屿特有物种西朱吉斯鹿鼠（P. sejugis）的两个种群中，变异水平显著较低且等位基因分布情况，与预期的重大奠基者效应相符，并表明这些种群之间缺乏近期的基因流动。基于共享等位基因频率的遗传距离的表型分析，一致产生了支持良好的树状图，这些树状图与鹿鼠物种组内先前得到证实的关系完全一致；从等位基因大小差异计算的遗传距离分析未得到此结果。微卫星数据不支持西朱吉斯鹿鼠应被视为与鹿鼠同种的假设，但产生了有力支持的西朱吉斯鹿鼠与基氏鹿鼠（P. keeni）之间的姐妹群关联。
