Lee R N, Jaskula J C, van den Bussche R A, Baker R J, Wichman H A
Department of Biological Sciences, University of Idaho, Moscow 83844, USA.
J Mol Evol. 1996 Jan;42(1):44-51. doi: 10.1007/BF00163210.
Mys is a retrovirus-like transposable element found throughout the genus Peromyscus. Several mys subfamilies identified on the basis of restriction site variation occur in more than one species. The distribution of these subfamilies is consistent with the accepted species phylogeny, suggesting that mys was present in the ancestor of Peromyscus and has been active through much of the evolution of this genus. Quantitative Southern blot analysis was used to examine the variability of subfamilies in P. leucopus and maniculatus. We found that subfamilies with phylogenetically narrow distributions were more variable in copy number both within and between species than subfamilies with a broader distribution. Taken together, our data suggest that mys has undergone multiple rounds of transposition since the peromyscine radiation, and that five subfamilies have been amplified during the evolution of the leucopus-maniculatus species complex.
Mys是一种在鹿鼠属中普遍存在的类似逆转录病毒的转座元件。基于限制性酶切位点变异鉴定出的几个Mys亚家族存在于多个物种中。这些亚家族的分布与公认的物种系统发育一致，表明Mys存在于鹿鼠属的祖先中，并且在该属的大部分进化过程中一直活跃。采用定量Southern印迹分析来检测白足鼠和鹿鼠中亚家族的变异性。我们发现，与分布较广的亚家族相比，系统发育分布较窄的亚家族在物种内和物种间的拷贝数变异更大。综合来看，我们的数据表明，自鹿鼠科辐射以来，Mys经历了多轮转座，并且在白足鼠 - 鹿鼠物种复合体的进化过程中，有五个亚家族得到了扩增。
