[因素对贯叶连翘愈伤组织生物量及金丝桃素合成量的影响]
[Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum].
作者信息
Xu Ming-shu, Huang Lu-qi, Chen Mei-lan, Ye He-chun, Li Guo-feng
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):921-3.
OBJECTIVE
To study the effect of several factors on the quantity of hypericin in H. perforatum callus.
METHOD
High efficiency liquid phase chromatography and plant tissue culture were applied.
RESULT AND CONCLUSION
When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
目的
研究多种因素对贯叶连翘愈伤组织中金丝桃素含量的影响。
方法
采用高效液相色谱法和植物组织培养技术。
结果与结论
当硝态氮与铵态氮比例为3:1时，愈伤组织生物量增加1.6倍，金丝桃素合成量上升。0.1 - 0.20 mg·L⁻¹的甘露糖可提高总金丝桃素含量。添加聚乙烯吡咯烷酮（PVP）或交联聚乙烯吡咯烷酮（PVPP）可促进愈伤组织生长和生物合成。
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