Xu M S, Huang L Q, Fu M H, Te H C, Li G F
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700.
Zhongguo Zhong Yao Za Zhi. 2001 Dec;26(12):813-5.
To induce the callus of H. perforatum and identify hypericin and pseudohypericin of its callus.
The callus was induced in different culture conditions, and active constituents were determined by HPLC.
The inductions of callus from different parts were discussed, the induction rate of the leaf axil being the highest. The MS basic medium with 4 micrograms.L-1 2,4-D and 0.2 microgram.L-1 6-BA was the best of all screened media. Hypericin in the callus is determined by HPLC.
诱导贯叶连翘愈伤组织并鉴定其愈伤组织中的金丝桃素和假金丝桃素。
在不同培养条件下诱导愈伤组织，采用高效液相色谱法测定活性成分。
讨论了不同部位愈伤组织的诱导情况，叶腋处愈伤组织诱导率最高。筛选出的培养基中，添加4微克·升⁻¹ 2,4 - D和0.2微克·升⁻¹ 6 - BA的MS基本培养基效果最佳。采用高效液相色谱法测定愈伤组织中的金丝桃素。
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