Favot Laure, Gillingwater Marc, Scott Caroline, Kemp Paul R
Department of Biochemistry, University of Cambridge, Building O, Downing Site, Tennis Court Road, Cambridge CB2 1QW, UK.
FEBS Lett. 2005 Jan 3;579(1):100-4. doi: 10.1016/j.febslet.2004.11.054.
Proteins containing RPEL motifs (e.g., MAL) are important in the regulation of gene expression by the actin cytoskeleton. Screening the ENSEMBL database for RPEL proteins identified four additional proteins that contain RPEL motifs and nuclear localisation sequences, three of which (RPEL-A, RPEL-B and RPEL-C) are expressed in adult mouse tissues with different expression profiles. The mRNAs encoding RPEL-B and RPEL-C were subject to alternative splicing. Expression of these genes in cells indicated that they had a marked effect on cell shape. Furthermore, when expressed with a nuclear localised actin all of the different forms became restricted to the nucleus.
含有RPEL基序的蛋白质（如MAL）在肌动蛋白细胞骨架对基因表达的调控中起着重要作用。在ENSEMBL数据库中筛选RPEL蛋白时，鉴定出另外四种含有RPEL基序和核定位序列的蛋白质，其中三种（RPEL-A、RPEL-B和RPEL-C）在成年小鼠组织中表达，具有不同的表达谱。编码RPEL-B和RPEL-C的mRNA发生了可变剪接。这些基因在细胞中的表达表明它们对细胞形状有显著影响。此外，当与核定位的肌动蛋白一起表达时，所有不同形式都局限于细胞核。
