Berdy G J, Abelson M B, Smith L M, George M A
Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.
Arch Ophthalmol. 1992 Apr;110(4):528-32. doi: 10.1001/archopht.1992.01080160106043.
Scanning electron microscopy was used to evaluate the corneal epithelium of rabbit eyes after administration of two preservative-free ocular lubricants, preservative-free artificial tear-1 (Hypotears PF) and preservative-free artificial tear-2 (Refresh), and 0.02% benzalkonium chloride. Animals were randomly assigned to either mild or exaggerated use regimens. A quantitative rating system was used to assess epithelial damage. With mild use, scanning electron microscopy revealed normal epithelial morphologic characteristics for both preservative-free artificial tear solutions (mean relative damage score, solution 1, 0.75 +/- 0.16; solution 2, 1.02 +/- 0.23), which were not significantly different from eyes treated with phosphate-buffered saline (1.38 +/- 0.38) or a mild dosage regimen of 0.02% benzalkonium chloride (1.20 +/- 0.12). Exaggerated use with preservative-free artificial tear solutions (solution 1, 1.31 +/- 0.21; solution 2, 1.35 +/- 0.08) induced minimal damage that was not different from control eyes treated with phosphate-buffered saline (1.26 +/- 0.13). Compared with an exaggerated use of 0.02% benzalkonium chloride (4.0 +/- 0.16), both preservative-free artificial tear solutions induced significantly less epithelial damage (P = .0001). These results suggest that with frequent-dosage regimens, preservation-free artificial tear solutions-1 and -2 are free of the toxic effects associated with preserved solutions.
使用扫描电子显微镜评估给予两种无防腐剂眼部润滑剂、无防腐剂人工泪液 -1（Hypotears PF）和无防腐剂人工泪液 -2（Refresh）以及0.02%苯扎氯铵后兔眼的角膜上皮。动物被随机分配到轻度或过度使用方案组。采用定量评分系统评估上皮损伤。轻度使用时，扫描电子显微镜显示两种无防腐剂人工泪液溶液的上皮形态特征正常（平均相对损伤评分，溶液1为0.75±0.16；溶液2为1.02±0.23），这与用磷酸盐缓冲盐水处理的眼睛（1.38±0.38）或0.02%苯扎氯铵的轻度剂量方案处理的眼睛（1.20±0.12）无显著差异。无防腐剂人工泪液溶液过度使用（溶液1为1.31±0.21；溶液2为1.35±0.08）引起的损伤极小，与用磷酸盐缓冲盐水处理的对照眼（1.26±0.13）无差异。与0.02%苯扎氯铵过度使用（4.0±0.16）相比，两种无防腐剂人工泪液溶液引起的上皮损伤均显著更少（P = .0001）。这些结果表明，在频繁给药方案下，无防腐剂人工泪液溶液1和2没有与含防腐剂溶液相关的毒性作用。
