Major T, Szarka K, Sziklai I, Gergely L, Czeglédy J
Clinic of Otorhinolarnygology and Head and Neck Surgery, Medical and Health Science Centre, University of Debrecen, 4012 Debrecen, Hungary.
J Clin Pathol. 2005 Jan;58(1):51-5. doi: 10.1136/jcp.2004.016634.
To determine the prevalence, type, physical state, and viral load of human papillomavirus (HPV) DNA in cases of head and neck cancer and recurrent respiratory papillomatosis (RRP).
The prevalence and type of HPV DNA was determined in 27 fresh frozen tissue specimens from patients with head and neck cancers and 16 specimens from 10 patients with RRP by MY09/MY11 and GP5+/GP6+ nested polymerase chain reaction (PCR) and subsequent restriction enzyme cleavage. The physical state of HPV DNA was analysed by E1, E2, and E1E2 specific PCRs and Southern blot hybridisation (SBH).
HPV DNA was detected in 13 of 27 cancers and 10 of 10 papillomas. Both low risk HPV-6 and HPV-11 and high risk HPV-16 were present in cancers in low copy numbers, whereas papillomas exclusively harboured low risk HPV-6 and HPV-11. E1E2 PCRs failed to determine the physical state of HPV in cancers except one case where HPV-6 DNA was integrated. In contrast to cancers, all papillomas showed the episomal state of HPV DNA and a relatively higher viral load.
Based on the prevalence, type, physical state, and copy number of HPV DNA, cancers and papillomas tend to show a different HPV DNA profile. The 100% positivity rate of low risk HPV types confirms the role of HPV-6 and HPV-11 in the aetiology of RRP.
确定头颈部癌和复发性呼吸道乳头状瘤病（RRP）病例中人乳头瘤病毒（HPV）DNA的患病率、类型、物理状态和病毒载量。
通过MY09/MY11和GP5+/GP6+巢式聚合酶链反应（PCR）及随后的限制性内切酶切割，测定27例头颈部癌患者新鲜冷冻组织标本和10例RRP患者的16份标本中HPV DNA的患病率和类型。通过E1、E2和E1E2特异性PCR及Southern印迹杂交（SBH）分析HPV DNA的物理状态。
27例癌症中有13例检测到HPV DNA，10例乳头状瘤中有10例检测到。低风险的HPV-6和HPV-11以及高风险的HPV-16在癌症中均以低拷贝数存在，而乳头状瘤仅含有低风险的HPV-6和HPV-11。除1例HPV-6 DNA整合的病例外，E1E2 PCR未能确定癌症中HPV的物理状态。与癌症不同，所有乳头状瘤均显示HPV DNA的游离状态和相对较高的病毒载量。
基于HPV DNA的患病率、类型、物理状态和拷贝数，癌症和乳头状瘤倾向于表现出不同的HPV DNA谱。低风险HPV类型的100%阳性率证实了HPV-6和HPV-11在RRP病因学中的作用。
